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KMID : 0386720080200030209
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Journal of the Korean Hip Society
2008 Volume.20 No. 3 p.209 ~ p.214
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Activation of Transcription Factor NF-κB and c-Jun/AP-1 by Various Compositions of Particles in Osteoclast Precursor Cells
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Kim Yong-Sik
Kwon Soon-Yong
Han Suk-Ku
Kwon Jae-Young
Choi Nam-Yong
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Abstract
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Purpose: This study was performed in order to investigate the effects of various particle preparations on NF-κB and c-Jun/AP-1 activity in osteoclast precursor cells.
Materials and Methods: Osteoclast precursor cells isolated from C57BL mice were treated with PMMA (polymethylmethacrylate) spheres, polystyrene, titanium particles, and retrieved metal particles from failed cementless total hip replacements. NF-κB and c-Jun/AP-1 DNA binding activities were analyzed using electrophoretic mobility shift assays (EMSA).
Results: Commercially available PMMA and polystyrene spheres routinely showed negativity on endotoxin assays, but titanium particles and retrieved metal particles consistently showed positivity. PMMA spheres, with a maximal response noted at 30 minutes with an optimal concentration of 0.6 mg/ml, were potent stimulator of NF-κB and c-Jun/AP-1 activity in osteoclast precursor cells. Other particles (polystyrene, titanium, metal retrievals) also activated transcription factor NF-κB and c-Jun/AP-1 compared to controls. Endotoxin removal from retrieved metal particles diminished the biologic effect by approximately 40%.
Conclusion: Particles of various compositions and sizes (PMMA, polystyrene, titanium, and retrieved metal particles) activated the NF-κB and c-Jun/AP-1 signaling pathways. This suggests that NF-κB and c-Jun/AP-1 may have important roles in the pathogenesis of periprosthetic osteolysis.
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KEYWORD
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Electrophoretic mobility shift assay, Transcription factor, NF-κB, c-Jun/AP-1, Particle, Osteoclast precursor cell
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